Nikon NiE - Bioimaging - Newcastle University

Modalities: upright, widefield

Overview

The Nikon NiE is a semi-automated microscope.

The Nikon NiE microscope can be used to view slides. The camera is an Andor Zyla 5.2.

The filters, condenser, motorized stage and z drive are all automated. This allows for multichannel, z stack imaging to be performed.

The system is run through Nikon’s versatile Elements software package. It also incorporates wizards to allow the automated capture and analysis of fluorescence images.

Imaging modes

Widefield fluorescence
Phase Contrast
XYZ (3D)

Specification

Here you can find the specifications for this microscope.

Objectives

Magnification	NA	Coverslip	Other Modes	Immersion
4	0.2			Air
10	0.45	0.17		Air
20	0.7	0.17		Air
40	0.95	0.17		Air
40	1.3	0.17		Oil
100	1.4	0.17		Oil

Filters (epi)

Cube	Example fluorophores	Excitation	Dichroic	Emission	Part Number
Nikon DAPI	DAPI, Hoechst	340-380	400	435-485
Nikon FITC	GFP, FITC, AF488	465-495	505	515-555
Nikon TRITC	TRITC	527-552	565	557-633
Nikon Cy3	Cy3	515-555	565	570-610
Nikon Cy5	Cy5, AF647	590-650	660	665-735